Prof. D. P. *****

***** Research

Office of the Dean

***ADRESS****

19 August 1992

Dear Dr. D. P. *****

Thank you for your letter dated July 13, 1992. We submit a manuscript entitled "Title. (MS:*****)" by **authors**. Enclosed please fined a original manuscript along with three copies and a copy on computer disk of revised version of the paper. The 3.5 inch floppy disk was formatted by Apple Macintosh computer, and the application used was Microsoft Word.

We believe that the paper has been much improved, Iargely as a result of the referee's many thoughtful comments. I would like to explain below how we have responded to each comment.

Answers to referee's comments

General comments:

We made clear the series resistances reflecting the access resistance through the perforated patch (p.6. 1.17).

The English style was corrected throughout the paper.

Specific Comments:

l 　"Is 32% of Icaffeine....to apamin and norepinephrine?"

The TEA-resistant current was not depressed by neither apamin ( ImicroM), and norepinephrine ( 100 microM). We concluded that the TEA-resistant current was not IAHP. We described the results and discussed in p. 10. l.5 and p.14. l. 20.

2. "It was not clear whether....."

We answered against your question as in p. lO. l.11.

3. "Is Icaffeine affected by charybdotoxin?"

Icaffeine Was depressed by charybdotoxin (0.2 microM) to 25-30 %. We described this result in p.9. l.20.

4. "Pg. 11 Iine 4...."

Our opinion was agreement with yours. We changed the sentences as in p.10. l.27.

5. "Pg. 12 Iine 4...."

Illustrate in Figure 6A was shown to explain the experimental protocol. The illustrate also shows full recovery of Icaffeine in the presence of 2 mM [Ca2+]o

6. "Pg. 13 botom..."

This information was obtained from our observation and we already mentioned the observation in annual meeting of Japanese Journal ***** (1992) . We cited the abstract in annual meeting of Japan. J. ***** (See reference 27)

"7. Pg. 16. Fig 16 seems to...

Thank you for your nice suggestion. We mentioned your suggestion as in p.11. l.22.

8. "It would have been nice...."

Thank you for your suggestion. Unfortunately, ruthenium red was membrane-inpermeant. We could not examine the effect of ruthenium red on Icaffeine, by using nystatin-perforated mode.

I hope our responses are satisfactory, and we look forward to hearing from you again. Thank you for your attentions and considerations in this matter.

Sincerely yours,