Dear Dr. ***,
Your manuscript no. ***, entitled:
Title,
has been evaluated by the referee(s), who raised some points that are detailed on the enclosed evaluation sheet(s).
We are of the opinion that this manuscript can be accepted for publication in the JOURNAL** after minor revision.
We would appreciate it if you would modify the manuscript according to the suggestions made by the referees. Also, we inform you that, if a checklist is included, the points herein should be taken care of.
Please reply to the comments of the referee(s ) and indicate which of the required changes have been made and where these changes are to be found in the revised manuscript (page and line). We also ask you to give the reasons that led you to reject some of the suggested changes.
Would you please send us the manuscript in duplicate, i.e. the entire revised manuscript and one copy thereof, including the complete set of illustrations and list of references for the manuscript and for the copy. Your reply to the comments made by the referee(s), also in duplicate, should accompany the manuscript.
Yours sincerely,
Referee's Comments
The authors report studies on kinetic characteristics of thrombin receptor-mediated responses, i.e., Ca2+- activated K+ currents, in rat megakaryocytes, using a rapid drug exchange system. Overall, the thrombin responses were compared with similar responses induced by activation of purinoceptors with ATP. It is shown that, in comparison to the ATP response, the thrombin-induced response is characterized by a long latency, a long washout time and a fast desensitization. In addition to thrombin, the megakaryocyte responses to trypsin and a synthetic thrombin receptor agonist peptide, were also studied. In general, this is a well performed study containing new interesting data which deserves publication.
Comments:
- Title: It should read "Kinetic characteristics of thrombin receptor-mediated responses in ratL/ megakaryocytes "
-References: There are four papers "Uneyama et al., 1993". Thus, they have to be defined as 1993a, /- 1993b, 1993c and 1993d, and accordingly also in the text. Similarly there are two papers "Vu et al., 1991 " .
-The full name of SBTI needs to be given.
-"TB" should not be used as an abbreviation for thrombin.
-Nakagawa et al., 1989 (.p. 3) or 1991 (List of References)?
-p. 3, Iine 5: "fibroblasts".
-p. 3, line 3 from bottom: "2.1. Preparation of megakaryocyte,
- p. 4, Iine 4 from bottom: "as reported before (Uneyama et al., 1992; 1993a)".
- p. 5, Iine 6 from bottom: The city where Peninsula is located is not "California".
-p. 7, Iine 13: "Spannagl et al., 1991," is not given in the List of References.
-p. 7, Iine 6 from bottom: Remove "also".
-p. 10, 1st para of Dicussion: It is mentioned that the thrombin-induced Ca2+-activated K+ current "partially depended on extracellular Ca2+". These data are neither shown nor mentioned in the Results section.
-p. 10, Iine 1 from bottom: "Jakobs & Aktories, 1988".
-p. 11, Iines 12-14: "Some lines of evidence suggest that both thrombin and ATP mobilize Ca2+ from intracellular stores via activation of the phosphatidylinositol cascade".
-Fig. 5 Iegend and p. 9: Thrombin was applied for 20 or 30 sec?
-Table 1 : A Iegend is missing, indicating the number of experiments as well as whether SD or SEM values are shown.
Referee's Comments
It is shown in this paper that thrombin activates a Ca2'-dependent K+ current in megakaryocytes. Although it is not proven beyond doubt that the current is an IKCa it probably is.
The phannacological results are convincing and the paper deserves publication. However, the authors need to corrcct frequent language errors. I have picked up some errors that are listed below but have probably missed many others.
P3 Para I Iine l a shouldbe an'
P3 para I line3 an should be a'
P3 Para I line4 fibloblasts should be fibroblasts
P3 Para I Iine 9 platelet should be p]atelets'
P3 Para I line 9 receptor shouId be receptors'
P3 Para I Iine 10 ce]1 should be cells'
P3 Para 2 Iine l "the" should be omitted:
P3 Para 2 Iine 12 "sec order needs rewording
.....
P12 para 4 line 3 ...